| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051161 |
| Chromosome: | chromosome 1 |
| Location: | 4590751 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAACAAATTGACATTTACGAGAGGCCA |
| Internal bar code: | CGGGATCTATCAGTTAGACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 99.5319 |
| LEAP-Seq n confirming: | 1701 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCAGAAGGGGATGAATA |
| Suggested primer 2: | CCCTCGTGTTGTCAACCTTT |