| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051163 |
| Chromosome: | chromosome 10 |
| Location: | 197502 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419100 | Hypothetical protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGGGGGCCGCATCAGGGTGCAGCTCCA |
| Internal bar code: | GGAAGCTGGGGGACCCAGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGTTAGAATGCGTTGCG |
| Suggested primer 2: | GGCTTCTGTCAATCAGCTCC |