| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051181 |
| Chromosome: | chromosome 11 |
| Location: | 2501852 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475750 | (1 of 1) PF04187 - Haem-binding uptake, Tiki superfamily, ChaN (Cofac_haem_bdg) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAATGGGCAATGGCCTAGTTGCTGTCG |
| Internal bar code: | GAGATCTCTTACGTTATGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 99.3939 |
| LEAP-Seq n confirming: | 164 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACAAGATGGGGCGTGAT |
| Suggested primer 2: | CCATACCAACACCAACACCA |