| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051185 |
| Chromosome: | chromosome 3 |
| Location: | 882545 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146607 | (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTGGGCACGGCCACAACCCGCTGCAGG |
| Internal bar code: | GACCGCAGTGGTACGCATGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.8161 |
| LEAP-Seq n confirming: | 2171 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCTCCTACGATCCAC |
| Suggested primer 2: | GAGACCCATGCAGGTTCACT |