| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051198 |
| Chromosome: | chromosome 17 |
| Location: | 2155968 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712900 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACTCGACCGTGTCCTTTAGGCCACCAA |
| Internal bar code: | AGAATGGGGCTGAGATGGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 2.38711 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 8301 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCCAACTCCAAAGCTGC |
| Suggested primer 2: | GCTGTCTTAGATGGGCTTGC |