Insertion junction: LMJ.RY0402.051218_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.051218
Chromosome:chromosome 2
Location:3173743
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095068 (1 of 1) PTHR15535//PTHR15535:SF18 - TRANSMEMBRANE PROTEIN 2-RELATED // COMMUNICATION MUTANT PROTEIN F-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGATACACCCAACGCCAGCTGTACAGCTC
Internal bar code:AGCTATACGTTGTGCACTAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:272
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCTTTAACAGCGCGTCC
Suggested primer 2:GCGACACATGATGGTAAACG