| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051226 |
| Chromosome: | chromosome 2 |
| Location: | 1413777 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083450 | (1 of 1) IPR000104//IPR002938 - Antifreeze protein, type I // FAD-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAATAGCCCGCCCGCTGATCGCAGCCGA |
| Internal bar code: | TCGGTACGCGTGGATTTCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 16.5266 |
| LEAP-Seq n confirming: | 4502 |
| LEAP-Seq n nonconfirming: | 22739 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCATTCTATCAGCCCTGG |
| Suggested primer 2: | GCTTGGATCATGGTTGTGTG |