| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051242 |
| Chromosome: | chromosome 1 |
| Location: | 5191812 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036100 | (1 of 1) K11790 - denticleless (DTL, CDT2, DCAF2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTCGACAGGCGCATGGCGTCCTGCCGT |
| Internal bar code: | GAAGCGGTGACCAGCCGTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 99.0615 |
| LEAP-Seq n confirming: | 6650 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCCAGTCATTGTTGTGC |
| Suggested primer 2: | CAGTGATGGTCAGTGGATGG |