Insertion junction: LMJ.RY0402.051257_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051257
Chromosome:chromosome 10
Location:5533216
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g459450 SUB10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAATCATCGAGGCCGCACCCGTAGCTGG
Internal bar code:GCACCGAACCGGGTGGGCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:645
LEAP-Seq percent confirming:96.5994
LEAP-Seq n confirming:8096
LEAP-Seq n nonconfirming:285
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GACGTCTAACACCGGCAAAT
Suggested primer 2:GGAATCGTAGGAAATGGCAA