Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.051267 |
Chromosome: | chromosome_16 |
Location: | 82490 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g695700 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTATAGTTCCCGACCAAGGAACGAAGCCAG |
Internal bar code: | CCTATATGTCCGGTGCATGATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 948 |
LEAP-Seq percent confirming: | 99.3333 |
LEAP-Seq n confirming: | 8493 |
LEAP-Seq n nonconfirming: | 57 |
LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAAACCGCAGTACCCTGTA |
Suggested primer 2: | CTTAGCCTTGCCAGAGATGG |