Insertion junction: LMJ.RY0402.051267_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051267
Chromosome:chromosome_16
Location:82490
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g695700 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTATAGTTCCCGACCAAGGAACGAAGCCAG
Internal bar code:CCTATATGTCCGGTGCATGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:948
LEAP-Seq percent confirming:99.3333
LEAP-Seq n confirming:8493
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAACCGCAGTACCCTGTA
Suggested primer 2:CTTAGCCTTGCCAGAGATGG