| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051272 |
| Chromosome: | chromosome 4 |
| Location: | 2704248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223375 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGAGGTGGTGGCGCGAGTTCCCGACG |
| Internal bar code: | TACGGGTCCAGAAAAATGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.0138 |
| LEAP-Seq n confirming: | 502 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGAGCAGGAGGAAGAG |
| Suggested primer 2: | TTCAGGATGAGGAATGGGAG |