Insertion junction: LMJ.RY0402.051280_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.051280
Chromosome:chromosome 6
Location:8121419
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g304876 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCTCAACGTCAGCACGGAGATATCCGA
Internal bar code:CTTTGACTCACCTAGGTACTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:10
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:121
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCTCCCCTCTGTTGCAG
Suggested primer 2:GGTGGTAATCGCGAAGTGTT