| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051282 |
| Chromosome: | chromosome 9 |
| Location: | 5818878 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402145 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAATGCTTGCACTTGCGTTTCGCTGGAC |
| Internal bar code: | GCGACGGTGGTCTACTACTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 89.0503 |
| LEAP-Seq n confirming: | 1716 |
| LEAP-Seq n nonconfirming: | 211 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGAGGTAGGGGAACTA |
| Suggested primer 2: | TACGGCCCATCATAGACCTC |