| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051292 |
| Chromosome: | chromosome 5 |
| Location: | 1623730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243353 | (1 of 14) PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTGGATCATTGCGGGGTTTCGTGTTTT |
| Internal bar code: | AATGCGAGACTTCGGCGGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 71.1073 |
| LEAP-Seq n confirming: | 30734 |
| LEAP-Seq n nonconfirming: | 12488 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGCTGTTGCTATGTTGC |
| Suggested primer 2: | TCAACCGAGATTCAGAACCC |