| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051303 |
| Chromosome: | chromosome 12 |
| Location: | 7447596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556400 | (1 of 1) K08066 - nuclear transcription factor Y, gamma (NFYC) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGGCTCAAAGGGTTCTAGTACCGTAAC |
| Internal bar code: | GGATCCCGGGGCGCCAGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.2629 |
| LEAP-Seq n confirming: | 8215 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGTATCTGCGAGTGGA |
| Suggested primer 2: | CTGCAAAGCAGGAGTAAGGG |