| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051306 |
| Chromosome: | chromosome 8 |
| Location: | 3722266 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376950 | MMP14 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCGCACTCGGCTGCCTGCGTGCGCATT |
| Internal bar code: | GGGCATGTCTCGAATCGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.2965 |
| LEAP-Seq n confirming: | 1835 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAGCTGAAGGTCTTGC |
| Suggested primer 2: | AGCGGGTGTGTATGTGTGAA |