| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051316 |
| Chromosome: | chromosome 3 |
| Location: | 7998516 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201700 | (1 of 1) PF15072 - Domain of unknown function (DUF4539) (DUF4539) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACAGCTCCAAGTGAGTAGGCCGCAGCT |
| Internal bar code: | ACCAAAGGGAGTATACCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 98.646 |
| LEAP-Seq n confirming: | 1020 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTGGTTGCTGTGATT |
| Suggested primer 2: | GACGCTGGCTTACTGTCTCC |