| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051351 |
| Chromosome: | chromosome 2 |
| Location: | 1576019 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084950 | GUK1 | Guanylate kinase; (1 of 1) K00942 - guanylate kinase (E2.7.4.8, gmk) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCTGGTGTGCGTGAGAGTGGAGAGGCA |
| Internal bar code: | CGGGCCCAAGTATCTTTCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.372 |
| LEAP-Seq n confirming: | 3323 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTCTTAGGAGCATCAG |
| Suggested primer 2: | GGTATGAGAGTGGCCGTGAT |