| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051355 |
| Chromosome: | chromosome 16 |
| Location: | 3034517 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665000 | UMP1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATCACAGTGATGACGAGGTGGTGGGTGC |
| Internal bar code: | TTAGGCGATGACACGGTACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 953 |
| LEAP-Seq percent confirming: | 99.2116 |
| LEAP-Seq n confirming: | 7047 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGGGTCGAAGTTCCCGT |
| Suggested primer 2: | TCATGCTGCCTGACAAAGTC |