Insertion junction: LMJ.RY0402.051356_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051356
Chromosome:chromosome 4
Location:2059083
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g219050 SRR10 Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCATGCGCGCACGCTCCCACCCATCAACA
Internal bar code:GTTACAACAGGGGTGTCACCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:763
LEAP-Seq percent confirming:99.6545
LEAP-Seq n confirming:3173
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACACTGTGCGTTGAGGT
Suggested primer 2:GCACACTTGGCACTGCTTTA