| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051361 |
| Chromosome: | chromosome 12 |
| Location: | 4025957 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517100 | (1 of 2) 5.5.1.6 - Chalcone isomerase / Chalcone--flavanone isomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTTCACAACAAAATGCGACGTTGCGAC |
| Internal bar code: | GCCTCTGTGATTTGGAAGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 99.6599 |
| LEAP-Seq n confirming: | 2930 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTAGTGGGTCAGCTGGTGG |
| Suggested primer 2: | GTCTTCTAATCGCCAAAGCG |