| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051379 |
| Chromosome: | chromosome 5 |
| Location: | 1659020 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243050 | TRX3,TRXf2,TRXF2 | (1 of 1) PTHR10438:SF258 - THIOREDOXIN F1, CHLOROPLASTIC-RELATED; Thioredoxin f2, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGCTACCGCCAAGGGCTACCGCCACG |
| Internal bar code: | TCGGGCCAGGTGACGCCTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 96.1749 |
| LEAP-Seq n confirming: | 2112 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGACAAGTGTTCCCCGGTC |
| Suggested primer 2: | TTGAGCCACACCCACAATTA |