Insertion junction: LMJ.RY0402.051391_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.051391
Chromosome:chromosome 3
Location:6699681
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g197513 (1 of 4) PF01679 - Proteolipid membrane potential modulator (Pmp3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTATACAGGATAAACTTGCTTCTTACA
Internal bar code:TACTTCCCTCGTTTCGCCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:950
LEAP-Seq percent confirming:99.3675
LEAP-Seq n confirming:1728
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTGGCAAGCATAGCATA
Suggested primer 2:CCCACTGGAATCCTTTGAGA