| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051400 |
| Chromosome: | chromosome 4 |
| Location: | 1163449 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215800 | (1 of 2) PF08457 - Sfi1 spindle body protein (Sfi1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAGCCCTTGCCCACCTGCGGCATGCGG |
| Internal bar code: | CACGCGACTGTACTACCTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1061 |
| LEAP-Seq percent confirming: | 99.4899 |
| LEAP-Seq n confirming: | 3901 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATGGTGTTGAGCTTGA |
| Suggested primer 2: | CCCTCTGTAACATCCGCATT |