Insertion junction: LMJ.RY0402.051401_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051401
Chromosome:chromosome 7
Location:5371318
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g350100 (1 of 1) IPR000104//IPR006311//IPR017956//IPR026741//IPR026937//IPR027417 - Antifreeze protein, type I // Twin-arginine translocation pathway, signal sequence // AT hook, DNA-binding motif // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGAATTGAGGATTGCTGCGGTGACGCCG
Internal bar code:GTGTGGCCGCTGATAGTCGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:593
LEAP-Seq percent confirming:32.753
LEAP-Seq n confirming:997
LEAP-Seq n nonconfirming:2047
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGCCATTCCTTTTATCTG
Suggested primer 2:CTCCCCTGTCATTGGACACT