Insertion junction: LMJ.RY0402.051409_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051409
Chromosome:chromosome 1
Location:604914
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g003487 (1 of 1) K13106 - pre-mRNA-splicing factor CWC26 (BUD13, CWC26) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCTCGCCCCTCCGCACCTTGGCGTCCG
Internal bar code:GGGTGGGTAGGGGGTCGGCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:138
LEAP-Seq percent confirming:99.2806
LEAP-Seq n confirming:138
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGCTTTTCCAAACATCC
Suggested primer 2:GTAAGGCTCGGCACGACAG