| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051411 |
| Chromosome: | chromosome 1 |
| Location: | 4905585 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034050 | (1 of 1) PTHR11751:SF428 - PROTEIN NKAT-1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGTAGGCTTCTTGGGCTCGCAAGCGC |
| Internal bar code: | ATCCGGGTCGGCAAGGTGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 96.7163 |
| LEAP-Seq n confirming: | 2209 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGATGAGAATCAAGGCCC |
| Suggested primer 2: | TAAGATCCTATGCAGCGCCT |