Insertion junction: LMJ.RY0402.051419_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051419
Chromosome:chromosome 11
Location:3005854
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g478456 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGGTCGAAAGCGGGTAGTGCCCGTACGC
Internal bar code:TGGACGATGGGGGGCCGCGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:772
LEAP-Seq percent confirming:92.5777
LEAP-Seq n confirming:16988
LEAP-Seq n nonconfirming:1362
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCTCCAGCTCCAGCATGT
Suggested primer 2:GGTTTCTTCCACGATTGCAT