Insertion junction: LMJ.RY0402.051437_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051437
Chromosome:chromosome 7
Location:3017767
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g333400 MMP23 Matrix metalloproteinase; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTCGGCGGGATCGGCGTCCGTCTGCACG
Internal bar code:GAATCGCGGCTTCAACGTACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:586
LEAP-Seq percent confirming:99.5671
LEAP-Seq n confirming:2990
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATCATATGACATCTGCG
Suggested primer 2:TGTTCCAGATCCCACAGTGA