Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.051437 |
Chromosome: | chromosome 7 |
Location: | 3017767 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g333400 | MMP23 | Matrix metalloproteinase; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCGGCGGGATCGGCGTCCGTCTGCACG |
Internal bar code: | GAATCGCGGCTTCAACGTACAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 586 |
LEAP-Seq percent confirming: | 99.5671 |
LEAP-Seq n confirming: | 2990 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCATCATATGACATCTGCG |
Suggested primer 2: | TGTTCCAGATCCCACAGTGA |