| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051458 |
| Chromosome: | chromosome 11 |
| Location: | 3718649 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482841 | (1 of 2) K11145 - ribonuclease III family protein [EC:3.1.26.-] (K11145) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTCAGGGTGCGCAAACGTTTTAATGGC |
| Internal bar code: | TTGACTTCCATGCCTATGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 95.5426 |
| LEAP-Seq n confirming: | 493 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGATATGACGCTCTCGGG |
| Suggested primer 2: | TTATGACTGTTGCCGCTGAG |