| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051460 |
| Chromosome: | chromosome 17 |
| Location: | 3301234 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723200 | FAP372 | Flagellar Associated EF Hand Protein 372 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGACTCGGCCTCCCAGGCCGCCACCGT |
| Internal bar code: | ATTTCTTGCTTCTTAGCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 99.6196 |
| LEAP-Seq n confirming: | 2619 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGAGCTGAATTGGAG |
| Suggested primer 2: | TTTACCAACAGCGCACAAAG |