| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051479 |
| Chromosome: | chromosome 1 |
| Location: | 194706 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001300 | ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACCGCTGTTGCCACTACTGCCGCCGAC |
| Internal bar code: | CGGGTGCCAACGGGGTGAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 42.0188 |
| LEAP-Seq n confirming: | 4833 |
| LEAP-Seq n nonconfirming: | 6669 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGTTGTTGATTGTGTCG |
| Suggested primer 2: | AATGTGGCGTCCTCCACTAC |