Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.051499 |
Chromosome: | chromosome_5 |
Location: | 2240089 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234661 | BCS1 | Ubiquinol:cytochrome c oxidoreductase biogenesis factor | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGAGCGGGACGAGCAGACAGAACATCATGT |
Internal bar code: | CCCCTTGATTTTGTGCTGCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 622 |
LEAP-Seq percent confirming: | 99.4543 |
LEAP-Seq n confirming: | 1458 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGGCCTTTCTACCTTCATC |
Suggested primer 2: | GTACGTGATCTTGGTGGCCT |