Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.051499 |
Chromosome: | chromosome_5 |
Location: | 2244073 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234662 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGTTTGCTTCACAGAACCGCCACCCGGAC |
Internal bar code: | GTTAAGGGCGAGCGATTCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 959 |
LEAP-Seq percent confirming: | 92.8362 |
LEAP-Seq n confirming: | 7581 |
LEAP-Seq n nonconfirming: | 585 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACCAATTGCCTTTTGTTGC |
Suggested primer 2: | AGGATGGCATTACCACTTGC |