Insertion junction: LMJ.RY0402.051511_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.051511
Chromosome:chromosome 12
Location:6104413
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g535800 (1 of 1) PF13191//PF13424 - AAA ATPase domain (AAA_16) // Tetratricopeptide repeat (TPR_12) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGAGGAATGGTAGAGGGCTGGGAGGGGG
Internal bar code:TTACGCGGCGGTTGTCTGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:6
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTCGCTGAATGAAACCA
Suggested primer 2:CGCGTGTTAGAGGGAGAAAG