Insertion junction: LMJ.RY0402.051512_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051512
Chromosome:chromosome 17
Location:7018108
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g746597 (1 of 2) K16296 - serine carboxypeptidase-like clade I [EC:3.4.16.-] (SCPL-I) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGAGTGCTGCCAGGTTGGCGGACAGCTT
Internal bar code:CTCTTTAGACCGACCAATTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:483
LEAP-Seq percent confirming:98.7787
LEAP-Seq n confirming:1375
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTAATGCCATGAGACGG
Suggested primer 2:CTGTTGCACGACAGGGAGTA