| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051537 |
| Chromosome: | chromosome 7 |
| Location: | 1815462 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325727 | (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTCGAACATGCGCTCCAGCGCCAGCCC |
| Internal bar code: | GGAAAATCCATGCCGTGTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 99.7947 |
| LEAP-Seq n confirming: | 486 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTCCGTGCTGATAGA |
| Suggested primer 2: | CCCATGTGCAGTGTTGTACC |