| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051541 |
| Chromosome: | chromosome 12 |
| Location: | 3112596 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499950 | CPL18 | (1 of 2) PF07819 - PGAP1-like protein (PGAP1); possible serine esterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGAAGTTGACGCAGCGCTACAGCCGT |
| Internal bar code: | AATGGCAGCTTTCCTGGGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 99.4393 |
| LEAP-Seq n confirming: | 11528 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCTACCTGCAACTCTCG |
| Suggested primer 2: | ACAGCCCCCTCACTACACAC |