Insertion junction: LMJ.RY0402.051558_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.051558
Chromosome:chromosome 12
Location:3019479
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g500850 (1 of 85) IPR009057 - Homeodomain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATGGACCCCACTGCTCCCATGGCCATGC
Internal bar code:AGTGCCATTTGGTTCGAACACC

Confirmation - LEAP-Seq

LEAP-Seq distance:652
LEAP-Seq percent confirming:93.6424
LEAP-Seq n confirming:1414
LEAP-Seq n nonconfirming:96
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCACAATGCCATGTTAG
Suggested primer 2:TCAGAAGTGACACACTCGCC