Insertion junction: LMJ.RY0402.051561_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.051561
Chromosome:chromosome 17
Location:5594340
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g737800 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCTCGCTTCCCCGACCCCGGACAAGG
Internal bar code:AGACAGGATAGGATTGTGGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:768
LEAP-Seq percent confirming:98.6694
LEAP-Seq n confirming:3411
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCACACATTCAGAGCCC
Suggested primer 2:GCGATGCAGGAGGTACTAGC