Insertion junction: LMJ.RY0402.051561_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.051561
Chromosome:chromosome 17
Location:5594348
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g737800 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCTTGAATGGCTGCGGTCCTTCGATCGC
Internal bar code:TCACTGCTTGTGGCGCACACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1235
LEAP-Seq percent confirming:98.8935
LEAP-Seq n confirming:2860
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCACACATTCAGAGCCC
Suggested primer 2:GCGATGCAGGAGGTACTAGC