Insertion junction: LMJ.RY0402.051563_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051563
Chromosome:chromosome 17
Location:562341
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g699750 CTL2 (1 of 1) IPR016187//IPR024616 - C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATATGTATCCGGACTGGATGTCACCTGC
Internal bar code:TCTCAAATTGAGCACACGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:410
LEAP-Seq percent confirming:99.6294
LEAP-Seq n confirming:8065
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGTACGGGCATTGTTTG
Suggested primer 2:GAATCAGGTCTTCAGGCTCG