| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051585 |
| Chromosome: | chromosome 3 |
| Location: | 8163494 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200450 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACACCCTCAATGATCCATCAGCCAACT |
| Internal bar code: | GCGCGGAGGGGATGGCGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 99.5433 |
| LEAP-Seq n confirming: | 13514 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGTCATGCTGATGGAA |
| Suggested primer 2: | CTACATCCGCTGGGAAACAT |