| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051615 |
| Chromosome: | chromosome 1 |
| Location: | 2024731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010864 | (1 of 1) K02836 - peptide chain release factor 2 (prfB); Homolog to bacterial PrfB peptide chain release factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGGAAGGCTAGGTAGCTGTCGGGGGTG |
| Internal bar code: | CGGCGGTCGCTCCACAAACCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 965 |
| LEAP-Seq percent confirming: | 99.6936 |
| LEAP-Seq n confirming: | 25057 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTTGTAGGAGTTGTTGC |
| Suggested primer 2: | CTGCCTTCCTAGCACACACA |