Insertion junction: LMJ.RY0402.051627_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051627
Chromosome:chromosome_16
Location:1617514
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g654150 FAP63 Flagellar Associated Protein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):AGTGGCGCCGAGCTCGGTCGGCATCCTGCA
Internal bar code:CATGTCCTCTCCAGGTCCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:158
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTCCCCCACGCTAATACC
Suggested primer 2:CGCCACCCATACAAAGAACT