| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051633 |
| Chromosome: | chromosome 16 |
| Location: | 4903099 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685700 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCATCGCCTTCAGCGCTTCGCACTTGC |
| Internal bar code: | CACAGCCGGACGAATTGCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 99.273 |
| LEAP-Seq n confirming: | 33182 |
| LEAP-Seq n nonconfirming: | 243 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGACTGCCTTGTGTCTG |
| Suggested primer 2: | GGAAGGCGGTCAGATCATAA |