| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051647 |
| Chromosome: | chromosome_6 |
| Location: | 7137146 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g297450 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCCAAACTGAAATCTTGCTGCCGAACGC |
| Internal bar code: | GTTCAATCACCGGGAAATACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 99.5501 |
| LEAP-Seq n confirming: | 2434 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGAAGTAGCGAGTCAGC |
| Suggested primer 2: | GGATCTGCACACGAGAGACA |