| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051681 |
| Chromosome: | chromosome 9 |
| Location: | 2421763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCTGGGGAAAGGGCTGTTTGGTCTGATG |
| Internal bar code: | AACGCGTGACGTGCGTGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 97.9644 |
| LEAP-Seq n confirming: | 6497 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCCGAAGCTGTATTAG |
| Suggested primer 2: | CACTAGCCGAGGGAGTTGAG |