| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051696 |
| Chromosome: | chromosome 8 |
| Location: | 2546995 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372300 | (1 of 1) IPR000104//IPR000620 - Antifreeze protein, type I // EamA domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCCCCCGGATTTTGTGGCACAGCGCAT |
| Internal bar code: | GGCGGGCAGGCGTAAACCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCGGGTGATATCGTGTG |
| Suggested primer 2: | TATGTCTCGGTGTCAGCTCG |