| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051719 |
| Chromosome: | chromosome 11 |
| Location: | 403967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467581 | (1 of 1) PTHR10071:SF177 - GATA-LIKE TRANSCRIPTION FACTOR ELT-6 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGAGTGTCGTCTGCCTTGTGTAGCTCC |
| Internal bar code: | TTGTGAGCGCCGGGCGGCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 98.9628 |
| LEAP-Seq n confirming: | 1145 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTCAGAGTCGGTTCGC |
| Suggested primer 2: | GTGTGTGGCGATGAAACAAC |